1998年11月7日 · VirD2 is one of the key Agrobacterium tumefaciens proteins involved in T-DNA processing and transfer. In addition to its endonuclease domain, VirD2 contains a bipartite C-terminal nuclear localization sequence (NLS) and a conserved region called omega that is important for virulence.

2020年1月23日 · Here, we generated a fusion between the Cas9 endonuclease and the Agrobacterium VirD2 relaxase (Cas9-VirD2). This chimeric protein combines the functions of Cas9, which produces targeted and...

VirD2包含强大的C端 核定位信号 序列，能引导T复合物穿过植物细胞质，通过核孔 进入细胞核。 进入细胞核以后，T-DNA将 靶向植物基因组进行整合，整合前会从超T复合体中 去除蛋白，⑥ VirE2从T链解离，而后 ⑦ T-DNA整合到植物 基因组。

2023年4月25日 · 农杆菌介导的遗传转化技术在植物、真菌、酵母中广泛使用，其拓扑异构酶VirD1能够辅助核酸内切酶VirD2识别并切割T-DNA的特异核苷酸序列。 研究人员将Cas9与VirD2蛋白融合形成Cas9-VirD2，在Cas9-VirD2融合蛋白基因编辑系统中加入特异DNA修复模板（包含编辑靶点的同源序列）和VirD1、VirE2蛋白，利用Cas9切割植物基因组形成双链断裂（DSB）和VirD2识别切割携带DNA修复模板的能力，使受VirE2保护的DNA修复模板在DSB位置进行同 …

1998年8月8日 · Agrobacterium, a plant pathogen, is capable to stably transform the plant cell with a segment of its own DNA called T-DNA (transferred DNA). This process depends, among others, on the specialized bacterial virulence proteins VirD1 and VirD2 that excise the T-DNA from its adjacent sequences.

The bacterial virulence protein VirD2 plays an important role in nuclear import and chromosomal integration of Agrobacterium-transferred DNA in fungal, plant, animal, and human cells. Here we show that in nuclei of alfalfa cells, VirD2 interacts ...

2014年3月13日 · The VirD2-binding protein (VBP) is a key cytoplasmic protein that recruits the VirD2-T-DNA complex to the VirD4-coupling protein (VirD4 CP) of the VirB/D4 T4SS apparatus.

The protein is shown to be the polypeptide VirD2, previously identified as a component of the T-DNA border endonuclease that initiates T-strand production. T-strands from an Agrobacterium strain expressing a virD2-lacZ fusion are bound to a protein of larger size than the wild-type protein and are immunoprecipitable by antibody to β-galactosidase.

1981年8月5日 · The VirD2 protein of Agrobacterium tumefaciens is essential for processing and transport of the T-DNA. It has at least three functional domains: a relaxase domain at the N terminus, a bipartite nuclear localization signal (NLS), …

Among the Ti-plasmid-encoded virulence (Vir) proteins that are required for T-strand formation and translocation to the recipient cell (Christie and Vogel 2000, Zhu et al. 2000), VirD2 is absolutely essential. Together with VirD1, VirD2 forms a relaxosome at the RB of the T-region, which initiates the formation of the T-strand.