question about the primer sequence #31 - GitHub
2020年3月12日 · The primers are actually are not used in the default profile HMM mode. The "universal tags" are used for PCR, but these primers are based on the reverse transcription …
GitHub - epi2me-labs/pychopper: cDNA read preprocessing
Pychopper v2 is a tool to identify, orient and trim full-length Nanopore cDNA reads. The tool is also able to rescue fused reads. The general approach of Pychopper v2 is the following: …
pychopper/pychopper/primer_data/cDNA_SSP_VNP.fas at master ...
A tool to identify, orient, trim and rescue full length cDNA reads - nanoporetech/pychopper
Nanopore-Mediated Sequencing of Circular RNA | SpringerLink
2024年2月22日 · A pool of 1–100 primers can be used and should target 30 nt downstream of the BSJ or downstream of the 5′ end of a conserved exon among different isoforms obtained from …
Nanopore sequencing of brain-derived full-length circRNAs …
2021年8月10日 · The need for gene-specific primers makes this technique ideal for delineating the complexity of circRNAs identified in global screens, from circRNA databases or …
mRNA vaccine quality analysis using RNA sequencing
2023年9月21日 · Here we describe a streamlined method to analyse mRNA vaccines and therapies using long-read nanopore sequencing. Compared to other industry-standard …
Nanopore sequencing of RNA and cDNA molecules in - PMC
Full-length cDNA reads containing strand-switching primer (SSP) and anchored oligo (dT) VN primer (VNP) in the correct orientation were identified using Pychopper (v.2.5.0) with standard …
Identification and trimming of full-length reads - microbepore
Full-length cDNA reads containing SSP and VNP primers in the correct orientation were identified using pychopper (v.2.5.0) with standard parameters using the default pHMM backend and …
Nanopore direct cDNA data analysis | https://databeauty.com
2022年10月20日 · Second round full-length cDNA reads identification applied to the unclassified direct cDNA reads with DCS-specific read rescue enabled (parameter -x). DCS109 can suffer …
pRSFDuet-1_VNp-LZ_VNp-LZ-hGH - Addgene
2023年2月3日 · Plasmid pRSFDuet-1_VNp-LZ_VNp-LZ-hGH from Dr. Dan Mulvihill's lab contains the inserts VNp-LZ-hGH and VNp-LZ and is published in Cell Rep Methods. 2023 Feb 2;3 …