2020年8月15日 · The mouse pancreatic ductal adenocarcinoma PAN02 was produced in C57BL/6 mice exposed to 3-methyl-cholanthrene. PAN02 is generally resistant to standard cancer therapeutics, thus providing an appropriately rigorous model for the development of clinically effective agents to treat aggressive and intractable pancreatic ductal adenocarcinoma.

2024年3月28日 · In searching for a pancreatic carcinoma cell that can be introduced into a mouse via simple injection, we found that Pan02, a murine ductal pancreatic adenocarcinoma derived from a pancreatic lesion of a C57BL/6 mouse, inoculated peritoneally can consistently produce pancreatic tumors.

We found that orthotopic Pan02 tumour-bearing mice responded poorly to gemcitabine, PD-L1 blockade and CTLA-4 blockade ( Figure 1 A-1C, study outlines in Supplementary Figure S1A). This allowed...

了解细胞相关信息和细胞处理方法。请准备好需要用到的培养基和相关试剂,尽量避免因. 进行镜检,拍照留档,方便后期对比。如发现细胞异常请及时与我们联系,如果三天内未与我们联系,则默认为收�. 了解细胞相关信息和细胞处理方法。请准备好需要用到的培养基和相关试剂,尽量避免因. 冻现象,请及时拍照并与我们联系。如果三天内未与我们联系,则默认为收�. 于-80°C,让管中液氮挥发,防止炸管�. 细胞至上述15 mL离心管,减�. 培养瓶或底面积相当的培养容器中。加入足量完全培养 …

2014年7月28日 · Methods: Pan02 cells were suspended in low-temperature Matrigel and injected into the parenchyma of pancreatic tails of C57BL/6 mice, with cells suspended in phosphate buffered saline (PBS) serving as a control. Primary and …

2017年7月1日 · Pan02 was derived from C57BL/6 mice given orthotopic 3-methyl-cholanthrene and is refractory to many standard chemotherapeutic agents. 1 Pan02 contains a loss of function mutation in the SMAD4 gene that is functionally similar to inactivating mutations in approximately 30% of human pancreatic cancers. 2

Panc02 细胞系是一种广泛使用的小鼠模型，用于研究胰腺导管腺癌 (PDAC)，这是最常见和最具侵袭性的胰腺癌形式。 Panc02 细胞最初源自 C57BL/6 小鼠化学诱导的胰腺肿瘤。 该细胞系在临床前研究中高度相关，因为它可以原位植入同基因小鼠体内，模仿自然肿瘤环境，并为 PDAC 的免疫反应和治疗耐药机制提供见解。 使用 Panc02 的研究为 PDAC 的免疫抑制微环境提供了重要的见解。 一项研究表明，Panc02 肿瘤被调节性 T 细胞 (Treg) 严重浸润，从而抑制抗肿瘤免疫反 …

1) Preparation: warm up the complete culture medium in 37°C water bath for 30 mins. Transfer the cryopreserved vial from liquid nitrogen to - 80°C freezer, and leave for several minutes to volatilize

胞生长状态良好时, 可进行细胞冻存。 贴壁细胞冻存时,弃去培养基后加入少量胰酶, 细胞变圆脱落后, 进行离心收集,1000RPM 条件下离心3-5 分钟,去除上清, 按冻存数量 .

PAN02小鼠胰腺导管腺癌细胞系最初由Corbett等人通过将携带3-甲基胆蒽的棉线植入 C57BL/6小鼠的胰腺，获得诱导的导管腺癌组织再进行系列皮下移植而建立。