2019年10月30日 · Gaps in knowledge about transmission patterns, evolution, and pathogenicity during infection have prompted a recent surge in genomic NTM research. Increased availability and affordability of whole genome sequencing (WGS) techniques provide new opportunities to sequence and construct complete bacterial genomes faster and at a lower cost.

In a post-genomic era, this review discusses the potential of whole genome sequencing (WGS) to predict antimicrobial susceptibilities in NTM and enable more focused treatment with reduced adverse events.

2020年4月23日 · However, recent studies using next-generation sequencing (NGS) have reported trans-continental spread of Mycobacterium abscessus among patients with cystic fibrosis. We investigated NTM genomes through NGS to examine transmission patterns in three pairs of co-habiting patients with NTM-PD who were suspected of patient-to-patient transmission.

2021年7月11日 · Mixed infection with multiple species of nontuberculous mycobacteria (NTM) is difficult to identify and to treat. Current conventional molecular-based methods for identifying mixed infections are limited due to low specificity. Here, we evaluated the utility of whole-genome sequencing (WGS) analysis to detect and identify mixed NTM infections.

2024年12月1日 · We evaluated the GenoType NTM-DR VER 1.0 assay using biobanked NTM isolates with whole genome sequence (WGS) data and control isolates (total n=285).

2021年7月11日 · Mixed infection with multiple species of nontuberculous mycobacteria (NTM) is difficult to identify and to treat. Current conventional molecular-based methods for identifying mixed infections are limited due to low specificity. Here, we evaluated the utility of whole-genome sequencing (WGS) analysis to detect and identify mixed NTM infections.

2021年10月27日 · WGS can identify all single nucleotide polymorphisms (SNPs) associated with resistance, as well as phylogenetic SNPs characteristic for individual NTM species [21]. In addition, WGS also contributes to the diagnosis of mixed NTM infections associated with two or more particular species [22].

2019年10月30日 · Gaps in knowledge about transmission patterns, evolution, and pathogenicity during infection have prompted a recent surge in genomic NTM research. Increased availability and affordability of whole genome sequencing (WGS) techniques provide new opportunities to sequence and construct complete bacterial genomes faster and at a lower cost.

2024年8月15日 · WGS of pathogenic microorganisms based on next-generation sequencing technology has been widely used in the detection of infectious diseases. It aids in diagnosing infections with two or more mixed NTM, and is also commonly used to identify rarely isolated species and understand resistance mechanisms by obtaining complete genome sequences [109].

2025年2月17日 · Targeted next-generation sequencing (tNGS) offers a promising alternative to line probe assays and whole genome sequencing (WGS), despite its higher cost. Amplification of specific gene regions lowers the input DNA requirements and mitigates the challenges associated with WGS in identifying NTM directly from clinical samples like sputum .