2021年11月12日 · Here, we demonstrate that WRN helicase ensures efficient restart and limits excessive degradation of stalled forks in BRCA2-deficient cancer cells. In vitro, WRN ATPase/helicase catalyzes fork...

2025年1月25日 · To identify signaling pathways dysregulated in WRN-deficient MSC, we generated WRN knockdown (WRN-KD) MSC in human umbilical cord-derived MSC (UC-MSC). We screened the five major protein phosphorylation pathways which include MAPK, AKT, Jak/Stat, NFκB and TGFβ pathways using ELISA-based protein array.

2019年3月3日 · Werner syndrome protein (WRN) is a RecQ enzyme involved in the maintenance of genome integrity. Germline loss-of-function mutations in WRN led to premature aging and predisposition to cancer. We evaluated synthetic lethal (SL) interactions between WRN and another human RecQ helicase, BLM, with DNA damage response genes in cancer cell lines.

敲除Wrn基因exon 5-8，建立Wrn基因敲除小鼠模型。 应用领域 ：衰老，细胞衰老；肿瘤研究 *使用本品系发表的文献需注明: Wrn-KO mice (Cat. NO. NM-KO-190902) were purchased from Shanghai Model Organisms Center, Inc..

Werner综合征（WS）蛋白（WRN）是DNA解旋酶RecQ家族中的一员，在DNA复制、转录、修复、重组和异染色质维持中发挥重要作用。 WRN基因的突变导致了WS的表现，这是一种以加速衰老为特征的疾病。

Werner's syndrome (WS) is a human disease with manifestations resembling premature aging. The gene defective in WS, WRN, encodes a DNA helicase. Here, we describe the generation of mice bearing a mutation that eliminates expression of the C terminus of the helicase domain of the WRN protein.

2021年6月1日 · 该研究在60个耐药性结直肠癌实验模型中进行了WRN基因敲除，证明WRN依赖性是耐药性结直肠癌的突出特征，显示了针对dMMR/MSI-H结直肠癌患者的WRN基因的治疗潜力， 靶向WRN基因有望帮助开发治疗耐药性结直肠癌的新型靶向疗法 。 在这项研究中，作者收集了迄今为止最大的dMMR结直肠癌临床前模型，包括来自原发肿瘤和转移病灶的60个独特模型（如癌细胞系和患者来源的3D器官培养）。 通过CRISPR Cas9和/或RNA干扰WRN、克隆形成试验、 …

Here we constructed an integrated experi-mental cascade, which contains both in vitro and in vivo assays, to conduct the high throughput hit-to-lead com-pound screen. WRN proteins of different length have been successfully purified and utilized to develop multiple biochemical assays such as unwinding assay and ATPase assay.

2023年10月26日 · Werner综合征（WS）蛋白（WRN）是DNA解旋酶RecQ家族中的一员，在DNA复制、转录、修复、重组和异染色质维持中发挥重要作用。 WRN基因的突变导致了WS的表现，这是一种以加速衰老为特征的疾病。

Werner syndrome RecQ helicase-IN-4 is a potent and orally active werner syndrome recQ helicase (WRN) inhibitor with an IC 50 value of 0.06 µM. Werner syndrome RecQ helicase-IN-4 shows antiproliferative activity.