2021年7月16日 · Gram-negative species couple polymerization with translocation across the periplasm and outer membrane and the master regulator of the system is the tyrosine autokinase, Wzc. This near atomic...

Expression levels of wzb, wzc and wzi were decreased in wza knockout strains. The antioxidant capacity of Wza knockout bacteria was only slightly decreased. Complementation of the wza gene returned the adhesion ability, antiserum complement killing ability, and mortality of G. mellonella and mic e to WT levels.

The structure of the Wzb–Wzc CDΔC complex determined here not only reveals key structural features that enable molecular recognition between these two species but also provides a mechanistic framework to understand how the assembly/disassembly cycle correlates to Y-cluster phosphorylation states .

EPS的合成及其输出受细菌Wza-Wzb-Wzc系统的调节。 Wzc表现出自磷酸化蛋白酪氨酸激酶活性，而Wzb是一种蛋白酪氨酸磷酸酶。 Wzb介导Wzc的去磷酸化。

2020年6月1日 · Wzc exhibits auto-phosphorylation protein tyrosine kinase activity, while Wzb is a protein tyrosine phosphatase. Wzb mediates dephosphorylation of Wzc. Dephosphorylated Wzc is required for the export of the EPS through porin Wza-Wzc complex. It shows that the interaction of Wzb with Wzc is critical for the export of EPS.

2019年1月23日 · The results obtained demonstrate that Wzc (Sll0923) and Wzb (Sll0328) are involved in the production of EPS, influencing both the amount and the composition of polymer(s). The absence of both Wzc and Wzb seems to redirect RPS production toward an alternative route.

EPS synthesis and its export is regulated by the bacterial Wza-Wzb-Wzc system. Wzc exhibits auto-phosphorylation protein tyrosine kinase activity, while Wzb is a protein tyrosine phosphatase. Wzb mediates dephosphorylation of Wzc. Dephosphorylated Wzc is required for the export of the EPS through porin Wza-Wzc complex.

In this biosynthesis pathway, Wza, Wzb, and Wzc homologues are required for surface expression of wild-type CPS or EPS. Multimeric complexes of Wza in the outer membrane are believed to provide a channel for polymer export; Wzc is an inner membrane tyrosine autokinase and Wzb is its cognate phosphatase.

2006年7月14日 · Low molecular weight protein-tyrosine phosphatases (LMW-PTPs) are small enzymes that ubiquitously exist in various organisms and play important roles in many biological processes. In Escherichia coli, the LMW-PTP Wzb dephosphorylates the autokinase Wzc, and the Wzc/Wzb pair regulates colanic acid production. However, the substrate recognition ...

Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography.