2021年7月8日 · The spliced form of X-box binding protein 1 (XBP1s) is an active transcription factor that plays a vital role in the unfolded protein response (UPR). Under endoplasmic reticulum (ER) stress, unspliced Xbp1 mRNA is cleaved by the activated stress sensor IRE1α and converted to the mature form encoding spliced XBP1 (XBP1s). Translated XBP1s ...

Under endoplasmic reticulum (ER) stress, unspliced Xbp1 mRNA is cleaved by the activated stress sensor IRE1α and converted to the mature form encoding spliced XBP1 (XBP1s). Translated XBP1s migrates to the nucleus and regulates the transcriptional programs of UPR target genes encoding ER molecular chaperones, folding enzymes, and ER-associated ...

2025年1月9日 · Here, we uncover unfolded protein response stress sensor inositol-requiring enzyme-1α (IRE1α) signaling in hematopoietic stem and progenitor cells (HSPCs) as a safeguard against myeloid...

2023年2月11日 · To better understand the genomic targets of XBP1s, we identified its genomic targets using Cleavage Under Targets and Release Using Nuclease (CUT&RUN) of a FLAG-epitope tagged XBP1s in RSV infection. CUT&RUN identified 7086 binding sites in chromatin that were enriched in AP-1 motifs and GC-sequences.

As the most conserved signaling branch of UPR, the IRE1-XBP1 pathway plays an important role in both physiological and pathological states, and its activity has a profound impact on disease progression and prognosis.

2023年1月9日 · IRE1α-XBP1 signaling is emerging as a central orchestrator of malignant progression and immunosuppression in various cancer types. Employing a computational XBP1s detection method applied...

Spliced XBP1 (XBP1s) is the direct downstream factor of IRE1α with activated RNase activity through splicing and removing 26 nucleotide intron from un-spliced XBP1 (XBP1u) (Figure 1), which is firstly identified as a key transcriptional regulator of major histocompatibility complex (MHC) class II in B cells in the early 1990s 3-5.

2021年9月30日 · Here we demonstrate that ERα controls the alternative splicing of XBP1 mRNA, a key component of the UPR pathway, and regulates the translation of stress response proteins such as eIF4G2 and MCL1 mRNAs. The ability of ERα to modulate these genes at the post-transcriptional level promotes cancer cell survival and sustains tamoxifen resistance.

We define a new role for XBP-1 in exerting selective translational control over high and sustained levels of immunoglobulin M (IgM) synthesis. XBP-1 −/− and XBP-1 +/+ primary B cells synthesize IgM at comparable levels at the onset of stimulation with lipopolysaccharide or CpG.

2022年11月8日 · Yang et al. demonstrate that tumor-intrinsic XBP1 dictates the production of cholesterol, which is secreted via small extracellular vesicles to drive immunosuppressive reprogramming by activating myeloid-derived suppressor cells. Blocking the IRE1α-XBP1-cholesterol axis reduces tumor growth.