Enriched DNA motifs of those regions revealed the XBP1s-binding motif contained the consensus sequence CACGT and NF-Y-binding motif. XBP1s target genes and their transcriptional regulatory networks are related to ER stress, the immune response, the …

2021年10月12日 · Using next‐generation sequencing (NGS) followed by bioinformatic analysis of XBP1-binding motifs, and validation using XBP1-specific silencing and quantitative real‐time PCR (qRT‐PCR), we defined an XBP1-dependent regulatory network and identified XBP1 as a repressor of both PUMA and IRE1 expression during the UPR. This approach not only ...

2024年12月25日 · XBP1 (X-Box Binding Protein 1) is a Protein Coding gene. Diseases associated with XBP1 include Major Affective Disorder 7 and Hematologic Cancer. Among its related pathways are Unfolded Protein Response (UPR) and …

2018年10月24日 · To further characterize the XBP1 regulome, we performed de novo motif discovery using HOMER to identify enriched DNA motifs within XBP1 binding regions. The top motif identified is the consensus sequence GCCACGT, which is almost identical to the human XBP1 binding motif defined in breast cancer cell lines (Fig. 4c ) [ 46 ].

2022年7月11日 · In particular, we identified Xbp1 as an evolutionarily conserved regulator of cell-fate determinations across different species. We demonstrated that Xbp1 transcriptional regulation is...

2021年7月8日 · The spliced form of X-box binding protein 1 (XBP1s) is an active transcription factor that plays a vital role in the unfolded protein response (UPR). Under endoplasmic reticulum (ER) stress, unspliced Xbp1 mRNA is cleaved by the activated stress sensor IRE1α and converted to the mature form e …

X-box binding protein 1 (XBP1) is a unique basic region leucine zipper (bZIP) transcription factor whose active form is generated by a nonconventional splicing reaction upon disruption of homeostasis in the endoplasmic reticulum (ER) and activation of the …

2019年2月19日 · Here we show that Fbw7, a substrate recognition component of the SKP1-Cullin-F-box-type E3 ligase, interacts with XBP1 in a phosphorylation-dependent manner, and facilitates XBP1 ubiquitination...

The non-canonical role of Xbp1 s in the fat body and its ability to promote longevity prompted us to further explore its function in this organ. Firstly, we performed motif enrichment analysis on promoters of genes differentially regulated by Xbp1 s in the fat body to find potential Xbp1 s …

As the most conserved signaling branch of UPR, the IRE1-XBP1 pathway plays an important role in both physiological and pathological states, and its activity has a profound impact on disease progression and prognosis.