Plasmid pLVX-XBP1 mNeonGreen NLS from Dr. David Andrews's lab contains the insert X-box binding protein 1 and is published in Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862. This plasmid is available through Addgene.

2022年6月22日 · Here, we have developed a single-molecule imaging approach to monitor the recruitment of individual XBP1 transcripts to the ER surface. Using this methodology, we confirmed that stable ER association of unspliced XBP1 mRNA is established through HR2 (hydrophobic region 2)-dependent targeting and relies on active translation.

2009年8月15日 · XBP1 is a key transcription factor that regulates the mammalian unfolded protein response. Its expression is regulated by unconventional mRNA splicing that is carried out by endonuclease IRE1 and a specific, as yet unknown, RNA ligase in response to the accumulation of unfolded proteins in the ER.

2006年1月1日 · The active form of the Xenopus X-box binding protein 1 (xXBP1) partially synergizes and partially antagonizes with BMP-4 signaling. xXBP1 overexpression inhibits mesoderm differentiation and formation of neural tissues. A functional knockdown promotes differentiation of lateral and dorsal mesoderm but not of ventral mesoderm and of neuroectoderm.

2024年12月25日 · XBP1 (X-Box Binding Protein 1) is a Protein Coding gene. Diseases associated with XBP1 include Major Affective Disorder 7 and Hematologic Cancer. Among its related pathways are Unfolded Protein Response (UPR) and …

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

LOCUS pLVX-XBP1 mNeonGreen NLS 9066 bp ds-DNA circular SYN 12-AUG-2021 DEFINITION ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen. ACCESSION . VERSION .

The spliced Xbp1 mRNA is translated by frameshift into an active transcription factor, XBP1s. Then, XBP1s upregulates the expression of UPR target genes, including ER chaperones, ERAD components, and lipid biosynthetic enzymes.

Plasmid pLHCX-XBP1 mNeonGreen NLS from Dr. David Andrews's lab contains the insert X-box binding protein 1 and is published in Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862. This plasmid is available through Addgene.

However, both XBP1U and XBP1S contain a nuclear localization sequence (NLS) required for nuclear translocation. In addition to the NLS, XBP1U contains two other subcellular localization determinants: a nuclear export sequence (NES) and a NES attenuator .