2007年4月13日 · Similar to the soluble XLF-Myc-His, GST-XLF increased the dimer ligation 7- and 11-fold at 3- and 10-fold ratios of GST-XLF to XRCC4·DNA ligase IV (Fig. 2D, lanes 1 versus 2 and 3). Bead-bound XLF-Myc-His was also tested in the ligation.

XLF (XRCC4-like factor), also called Cernunnos, was originally identified as the protein defective in a subset of RS-SCID patients who, in addition to immunodeficiency and radiosensitivity, displayed growth retardation, microcephaly, and lymphopenia.

MAb Anti GST-XL665 is an IgG2a raised against Glutathione S-transferase labeled with XL665. It has been shown to react with GST-tagged fusion proteins from a large number of expressing vectors.

The identification of XLF by a yeast two-hybrid screen for XRCC4 interactors suggested that XLF might functionally cooperate with the XRCC4-DNA Ligase IV complex. To begin to explore this possibility, we generated a glutathione S-transferase (GST)-XLF fusion protein in bacteria, purified this, and then used it in protein interaction studies.

2015年10月1日 · For expression and purification of Glutathione S-transferase (GST) tagged XLF protein, Escherichia coli BL21 (DE3) cells were transformed with pGEX6-GST-XLF plasmid. The GST-XLF protein was induced with isopropyl-β-D-1-thiogalactopyranoside. The cells were harvested and lysed, and cleared lysate was loaded onto a GSTrap HP column (GE ...

2008年10月1日 · GST-XLF 240–299 was immobilized on GST-beads and phosphorylated by DNA-PK as above, except that reactions contained 800 μg of GST-XLF 240–299 and 170 μg of purified DNA-PK complex. Phosphorylation reactions contained 0.25 mM ATP, while mock phosphorylation contained DNA-PKcs, Ku, DNA but no ATP.

Here, we report that Akt phosphorylates XLF at Thr181 to trigger its dissociation from the DNA ligase IV/XRCC4 complex, and promotes its interaction with 14-3-3β leading to XLF cytoplasmic retention, where cytosolic XLF is subsequently degraded by SCF β …

2008年12月4日 · We propose a model in which XLF, by in situ recharging DNA ligase IV after the first ligation event, promotes double stranded ligation by a single LX complex. DNA non-homologous end-joining (NHEJ) is the major mechanism for the repair of radiation induced DNA double strand breaks (DSBs) in mammalian cells.

Polyclonal Antibody for studying XLF. Validated for IP, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, XLF Antibody (CST #2854) is ready to ship.

To directly test the hypothesis that formation of an XRCC4/XLF complex inhibits DNA binding by XRCC4 and XLF, we coexpressed C-terminally his-tagged XRCC4 (XRCC4-his 6) and N-terminally GST-tagged XLF (GST-XLF) in E. coli, used Ni-NTA and glutathione sepharose affinity chromatography in tandem to isolate complexes that contain XRCC4-his 6 and ...