2007年4月13日 · Similar to the soluble XLF-Myc-His, GST-XLF increased the dimer ligation 7- and 11-fold at 3- and 10-fold ratios of GST-XLF to XRCC4·DNA ligase IV (Fig. 2D, lanes 1 …

XLF (XRCC4-like factor), also called Cernunnos, was originally identified as the protein defective in a subset of RS-SCID patients who, in addition to immunodeficiency and radiosensitivity, …

MAb Anti GST-XL665 is an IgG2a raised against Glutathione S-transferase labeled with XL665. It has been shown to react with GST-tagged fusion proteins from a large number of expressing …

The identification of XLF by a yeast two-hybrid screen for XRCC4 interactors suggested that XLF might functionally cooperate with the XRCC4-DNA Ligase IV complex. To begin to explore this …

2015年10月1日 · For expression and purification of Glutathione S-transferase (GST) tagged XLF protein, Escherichia coli BL21 (DE3) cells were transformed with pGEX6-GST-XLF plasmid. …

2008年10月1日 · GST-XLF 240–299 was immobilized on GST-beads and phosphorylated by DNA-PK as above, except that reactions contained 800 μg of GST-XLF 240–299 and 170 μg …

Here, we report that Akt phosphorylates XLF at Thr181 to trigger its dissociation from the DNA ligase IV/XRCC4 complex, and promotes its interaction with 14-3-3β leading to XLF …

2008年12月4日 · We propose a model in which XLF, by in situ recharging DNA ligase IV after the first ligation event, promotes double stranded ligation by a single LX complex. DNA non …

Polyclonal Antibody for studying XLF. Validated for IP, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, XLF Antibody (CST #2854) is ready to ship.

To directly test the hypothesis that formation of an XRCC4/XLF complex inhibits DNA binding by XRCC4 and XLF, we coexpressed C-terminally his-tagged XRCC4 (XRCC4-his 6) and N …