2007年4月13日 · Similar to the soluble XLF-Myc-His, GST-XLF increased the dimer ligation 7- and 11-fold at 3- and 10-fold ratios of GST-XLF to XRCC4·DNA ligase IV (Fig. 2D, lanes 1 versus 2 and 3). Bead-bound XLF-Myc-His was also tested in the ligation.

MAb Anti GST-XL665 is an IgG2a raised against Glutathione S-transferase labeled with XL665. It has been shown to react with GST-tagged fusion proteins from a large number of expressing vectors.

The identification of XLF by a yeast two-hybrid screen for XRCC4 interactors suggested that XLF might functionally cooperate with the XRCC4-DNA Ligase IV complex. To begin to explore this possibility, we generated a glutathione S-transferase (GST)-XLF fusion protein in bacteria, purified this, and then used it in protein interaction studies.

Here, we report that Akt phosphorylates XLF at Thr181 to trigger its dissociation from the DNA ligase IV/XRCC4 complex, and promotes its interaction with 14-3-3β leading to XLF cytoplasmic retention, where cytosolic XLF is subsequently degraded by SCF β …

2015年10月1日 · For expression and purification of Glutathione S-transferase (GST) tagged XLF protein, Escherichia coli BL21 (DE3) cells were transformed with pGEX6-GST-XLF plasmid. The GST-XLF protein was induced with isopropyl-β-D-1-thiogalactopyranoside. The cells were harvested and lysed, and cleared lysate was loaded onto a GSTrap HP column (GE ...

2008年10月1日 · GST-XLF 240–299 was immobilized on GST-beads and phosphorylated by DNA-PK as above, except that reactions contained 800 μg of GST-XLF 240–299 and 170 μg of purified DNA-PK complex. Phosphorylation reactions contained 0.25 mM ATP, while mock phosphorylation contained DNA-PKcs, Ku, DNA but no ATP.

2008年12月4日 · We propose a model in which XLF, by in situ recharging DNA ligase IV after the first ligation event, promotes double stranded ligation by a single LX complex. DNA non-homologous end-joining (NHEJ) is the major mechanism for the repair of radiation induced DNA double strand breaks (DSBs) in mammalian cells.

2008年9月9日 · Antibodies directed against XRCC4 (right) or GST (left) were used to IP XRCC4 and GST-XLF, respectively. The input fraction (Input), immunoprecipitated complexes (IP) and control IPs (C) of 10% were resolved on SDS–PAGE, subject to western transfer and XRCC4 and GST-XLF were detected as indicated.

XLF (XRCC4-like factor), also called Cernunnos, was originally identified as the protein defective in a subset of RS-SCID patients who, in addition to immunodeficiency and radiosensitivity, displayed growth retardation, microcephaly, and lymphopenia.

Polyclonal Antibody for studying XLF. Validated for IP, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, XLF Antibody (CST #2854) is ready to ship.

2022年6月11日 · 使用 GST 下拉和免疫共沉淀实验的基因功能，Ahnesorg 等人（2006） 发现 XL4 在体外和体内与 XRCC4 和 LIG4（601837） 相互作用。 小干扰 RNA 介导的 XLF 下调导致显着的放射敏感性和对引起 DSB 的药物的敏感性。

Construction of GST-XLF and XLF-Myc-His-Expressing Plasmids —Full-length human XLF cDNA was amplified by recombinant Pfu DNA polymerase (Stratagene, catalog #600154) using a human thymus matchmaker cDNA library (Clontech, catalog #HL4057AH) as the template (primers are available upon request).

2015年6月1日 · We found that human TDP1 stimulated DNA binding by XLF and physically interacted with XLF to form TDP1:XLF:DNA complexes. TDP1:XLF interactions preferentially stimulated TDP1 activity on dsDNA as compared to ssDNA. TDP1 also promoted DNA binding by Ku70/80 and stimulated DNA-PK activity.

Immunoblot (IB) of whole cell lysates (WCLs) derived from U2OS cells depleted of PTEN by two independent shRNAs (shGFP as a negative control). B-C. PTEN-depleted cells were more sensitive to DNA damage.

2008年10月1日 · GST-XLF 240–299 was immobilized on GST-beads and phosphorylated by DNA-PK as above, except that reactions contained 800 μg of GST-XLF240-299 and 170 μg of purified DNA-PK complex. Phosphorylation reactions contained 0.25 mM ATP, while mock phosphorylation contained DNA-PKcs, Ku, DNA but no ATP.

2022年9月10日 · xlf投資風險報酬走勢特性. 最大回檔風險：約-78.68%回檔. xlf於1998年成立，最大的回檔在2008年金融海嘯-78.68%，因為金融股對利率波動很敏感，當經濟衰退時容易受到影響，像是2008年金融海嘯時，金融業股價重挫，甚至有些公司可能也發不出股利。

2015年2月2日 · Here we report that Akt phosphorylates XLF (XRCC4 like factor, also called NHEJ1) at T181, to dissociate XLF from the XRCC4 (X-ray repair cross-complementing protein 4)/DNA ligase IV (LIG4) complex and subsequently triggers XLF cytoplasmic translocation, leading to XLF ubiquitination by SCF β-TRCP in a CKI-dependent manner. Together, our ...

分析SPDR Select Sector Fund - Financial (XLF)：检查其资产管理规模并研究回报、股息、资金流向和其他关键统计数据。

2015年2月19日 · Here we report that Akt phosphorylates XLF (XRCC4 like factor, also called NHEJ1) at T181, to dissociate XLF from the XRCC4 (X-ray repair cross-complementing protein 4)/DNA ligase IV (LIG4) complex and subsequently triggers XLF cytoplasmic translocation, leading to XLF ubiquitination by SCF β-TRCP in a CKI-dependent manner. Together, our ...

5 天之前 · 致力于整合全球宏观数据，试图发觉景气循环的蛛丝马迹，相信所有金融商品的投资决策都应回归基本面。 期待协助大家找到属于自己的投资地图!