2020年2月7日 · Here, we show that TIP60, also known as KAT5, a haplo-insufficient tumor suppressor, directly acetylates XPF at Lys911 following UV irradiation or treatment with mitomycin C and that this...

Despite the essential function of the XPF/ERCC1 complex for the human organism, we successfully generated a viable immortalised human XPF knockout cell line with complete loss of XPF using the CRISPR/Cas9 technique in fetal lung fibroblasts (MRC5Vi cells).

2021年9月28日 · We show that, during class switch recombination (CSR) in mature mouse B cells, the structure-specific endonuclease complex XPF-ERCC1SLX4, although not required for normal CSR, represents a nucleotide-excision-repair …

6 天之前 · We developed original cell models with CRISPR-Cas9-mediated knock-out of SLX4 and/or XPF using five different cell lines (A549, NCI-H1703, COLO-357, HT-29 and HEK-293 T), and performed characterization with cell biology experiments including migration assays, drug sensitivity testing, cell proliferation assessment and Western blots for relevant ...

2022年8月8日 · Here, we study the role of mouse SLX4 in HR triggered by a site-specific chromosomal DNA–protein replication fork barrier formed by the Escherichia coli -derived Tus– Ter complex. We show that...

2019年3月3日 · HCT116 constitutively suppressed APE by shRNA was used to generate a XPF mutant by CRISPR/Cas9 (Supplementary Figure 3C). The XPF inactivation in APE-suppressed HCT116 did not alter the cellular sensitivity to gemcitabine. We conclude that XPF and APE are genetically epistatic in the repair of gemcitabine-induced DNA damage.

2025年3月13日 · CRISPR/Cas9-mediated generation of trul-1 null allele For CRISPR/Cas9-mediated targeting we used the following sequence (which includes the PAM site) in exon 1 of trul-1 : GGTACCGAAGAATGGATTTGTGG ...

SLX4 and XPF are two proteins involved in DNA repair, but very little is known about their potential roles in other processes of cancer cell biology. We developed original cell models with CRISPR-Cas9-mediated knock-out of SLX4 and/or XPF using five different cell lines (A549, NCI-H1703, COLO-357, H …

XPF-1 C-terminally tagged with GFP using CRISPR-Cas9-medi-ated homology-directed repair. Knockin animals revealed that XPF-1 is expressed ubiquitously in nuclei of different tissues, including neurons, muscles, hypodermis, and intestine, as well as germ cells and embryos (Figure 1A). To be able to study

2017年1月27日 · Despite the essential function of the XPF/ERCC1 complex for the human organism, we successfully generated a viable immortalised human XPF knockout cell line with complete loss of XPF using the CRISPR/Cas9 technique in fetal lung fibroblasts (MRC5Vi cells). These cells showed a markedly increased sensitivity to UVC, cisplatin, and psoralen ...