YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris (P. pastoris) and Saccharomyces cerevisae (S. cerevisae) yeast strains. Each bottle of Gibco™ YPD Broth is filter-sterilized and ready-to-use in ergonomic Gibco™ media bottles with angled openings for simplified pipetting. For research use only.

2013年1月1日 · While all strains are capable of growth on ‘rich’ media (YPD), media contents can be altered to detect auxotrophic phenotypes, drug sensitivities, and metabolic capabilities, as well as to induce cell fate changes. The most commonly used yeast media are listed here.

Reagent Final concentration; Yeast extract 10 g/L: Glycerol: 20 mL/L: Peptone 20 g/L: Agar (for solid medium) 20 g/L: Autoclave the medium. Store at 4°C (this is stable for at least 6 mo).

Buffer and Media YPG medium Materials • 20 g Peptone • 10 g Yeast Extract • 20 mL Glycerol • 980 mL Double distilled water Procedure 1. Mix all materials in a flask filled with 1 L of double distilled water. 2. Autoclave 20 min, 121°C.

I. Yeast Media Sets (Table 1) are specifically designed for Matchmaker® Gold Systems. II. Yeast Media Pouches (Table 2) are ready-to-go, preformulated pouches; just add water and autoclave. No measuring, mixing, or pH adjustments are required. Each pouch makes 0.5 L of media, with or without agar. III.

2016年1月8日 · Basic Media . YPD/YPG . 500mL 600mL 1L 1.2L B-Peptone 10g 12g 20g 24g Yeast Extract 5g 6g 10g 12g . D. extrose / G. alactose 1 0 g 12 g 20 g 24 g ... Nat plates: pH with 2.5mL of 1:10 HCl/500mL media before autoclaving . Benomyl plates: Typically 15 µ g/mL, add 15mg into 1mL DMSO, add entire volume to 1L plates after autoclaving

YPG MEDIUM. Adjust the pH to 6.0 with dilute HCl. Solid media may be prepared by adding 15g/l agar.

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Media for S. cereviseae - YPD ( yeast extract p eptone d extrose): 1% yeast extract 10 g 2% peptone 20 g 2% agar 20 g 2% dextrose (glucose) 20 g H 2O to 1 l - YPG ( yeast extract peptone glycerol): 1% yeast extract 10 g 2% peptone 20 g 3% (v/v) glycerol 30 ml 2% agar 20 g H 2O to 1 l - YPAc ( yeast extract p eptone ac etate): 1% KOAc 10 g

Recipe for the preparation of YPG media.