The synthesis of QA by quinate/shikimate dehydrogenase (YdiB, ydiB) has been previously proposed; however, the precise role for this enzyme in the production of QA in engineered …

2003年5月23日 · Escherichia coli expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and a putative enzyme YdiB. Here we characterize YdiB as a dual specificity …

用于sh草酸（SA）生产的工程化大肠杆菌的培养导致奎尼酸（QA）和脱氢shi草酸（DHS）的合成，降低了SA的收率并损害了下游工艺。 先前已经提出了奎宁酸/ shi草酸脱氢 …

Escherichia coli expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and a putative enzyme YdiB. Here we characterize YdiB as a dual specificity quinate/shikimate …

2003年5月23日 · In this article, we report the crystal structure of the YdiB protein, which is one of the two shikimate 5-dehydrogenase orthologues in E. coli (12). It is common to find multiple …

莽草酸是芳香族氨基酸合成中的重要中间代谢物，可用于多种药物的化学合成．在代谢工程理论的指导下，构建积累莽草酸的高产菌株．通过构建敲除编码莽草酸激酶的基因aroL 与aroK的菌 …

结果表明:与大肠杆菌野生型BW25113 (WT)相比, ydiB 基因敲除菌株 ( ydiB)仅对氨基糖苷类抗生素耐受,并且发现过表达菌株BW25113-PCA24N (ydiB)能恢复对庆大霉素的敏感性。 因此,证明 …

Here, we report a study of Bacillus subtilis YdiB, which belongs to an uncharted class of bacterial P-loop ATPases. Precise deletion of the ydiB gene yielded a mutant with much reduced …

2003年5月23日 · We present here the 2.3-A crystal structure of the Escherichia coli YdiB protein, an orthologue of shikimate 5-dehydrogenase. This enzyme catalyzes the reduction of 3 …

2016年11月18日 · We report the effect of the inactivation or the overexpression of ydiB in E. coli strain PB12.SA22 on SA, QA, and DHS production in batch fermentor cultures.