Yeast GFP Fusion Localization Database
The YeastGFP database of global analysis of protein localization studies in the budding yeast, S. cerevisiae, was originally designed and built by the laboratories of Erin O'Shea and Jonathan …
Yeast GFP Clone Collection | Thermo Fisher Scientific - US
The Yeast GFP Clone Collection is a S. cerevisiae yeast strain collection expressing full-length ORFs containing an Aequorea victoria GFP (S65T) tag (Tsien, 1998) at the C-terminus end. …
Global analysis of protein localization in budding yeast | Nature
2003年10月16日 · By creating a GFP-tagged yeast strain collection and database that covers three-quarters of the proteome and over two-thirds of previously unlocalized proteins, we have …
Expression of varied GFPs in Saccharomyces cerevisiae : codon
2016年10月26日 · Here, we expressed GFPs from several sources in codon-optimized and non-codon-optimized forms in the yeast Saccharomyces cerevisiae, which represents an ideal …
Use of green fluorescent protein in living yeast cells
2002年1月1日 · This chapter discusses the use of green fluorescent protein (GFP) in living yeast cells. Although protein tags have been used for decades, the introduction of Aequorea victoria …
The Yeast GFP Clone Collection is a S. cerevisiae yeast strain collection expressing full-length ORFs containing an Aequorea victoria GFP (S65T) tag (Tsien, 1998) at the C-terminus end. …
Visualizing protein dynamics in yeast with green fluorescent protein
2000年1月1日 · The use of the Aequoreu Victoria green fluorescent protein (GFP) as a real-time molecular tag to study protein localization and dynamics has had a major impact in yeast cell …
Proteome-wide screens in Saccharomyces cerevisiae using the yeast GFP ...
The yeast GFP collection, an elegant and unique strain set, is ideal for studying both protein localization and abundance across the proteome ( http://yeastgfp.yeastgenome.org/ ). In this …
GFP FIXATION in Yeast
GFP FIXATION in Yeast : Fixation Technique 1. Spin cells in eppendorf and remove supe 2. Add 100 microliters of paraformaldehyde and vortex 3. incubate at RT for 15’ ...
Green fluorescent protein in Saccharomyces cerevisiae: real …
2000年10月20日 · Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was …
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