2013年5月27日 · Overexpressed Salmonella typhimurium YhjH (STM3611) enhances motitility, counteracts cellulose biosynthesis and the rdar phenotype, and mediates cyclic-di-GMP degradation. YhjH contains a putative cyclic-di-GMP phosphodiesterase EAL domain.

2013年5月24日 · YciR acts as a connector between modules I and II and functions as a trigger enzyme: its direct inhibition of the DGC YdaM is relieved when it binds and degrades c‐di‐GMP generated by module I. As a consequence, YdaM then generates c‐di‐GMP and—by direct and specific interaction—activates MlrA to stimulate csgD transcription.

2021年5月14日 · YhjH is a phosphodiesterase that degrades bis-(3’5’)-cyclic dimeric guanosine monophosphate (c-di-GMP) and has been shown to play a role in bacterial stress response by influencing biofilm formation.

2021年1月27日 · In the presence of nitrate, YhjH expressing cells formed significantly lower biofilm than the vector control whereas cells expressing YhjH E136A formed statistically significantly higher...

2010年4月9日 · E. coli and Salmonella have multiple c-di-GMP cyclases and phosphodiesterases, yet absence of a specific phosphodiesterase YhjH impairs motility in both bacteria. yhjH mutants have elevated c-di-GMP levels and require YcgR, a c-di-GMP-binding protein, for motility inhibition. We demonstrate that YcgR interacts with the flagellar switch-complex ...

2024年2月2日 · BolA downregulates the c -di-GMP pathway gene yhjH to inhibit motility. The generation of multicellular behavior enhances the stress adaptability, antibiotic resistance, and pathogenic potential of Salmonella enterica serovar Typhimurium (S. Typhimurium), which is challenging for its prevention and control.

2006年10月13日 · The yhjH mutation is expected to result in elevated c-di-GMP levels because YhjH is a c-di-GMP phosphodiesterase that strongly affects swimming motility in E. coli and Typhimurium (17, 23, 27, 28). We therefore tested the effect of the ycgR knockout in the yhjH mutant background.

最后，LacZ 基因报告显示 BolA 分别促进和抑制yeaJ和yhjH的表达。 综上所述，BolA主要通过促进 yeaJ 的表达来提高细胞外多糖的含量，从而增强生物膜的形成。

2017年8月22日 · While most EAL domain proteins contain additional, usually N-terminal, domains, there is a distinct family of proteins with stand-alone EAL domains, exemplified by Salmonella enterica serovar Typhimurium proteins STM3611 (YhjH/PdeH), a c-di-GMP-specific phosphodiesterase, and the enzymatically inactive STM1344 (YdiV/CdgR) and STM1697, which ...

2024年2月2日 · Further mechanistic analysis demonstrated that BolA affected the expression of the C-di-GMP pathway genes yeaJ and yhjH, which influenced the generation of multicellular behavior. In terms of biofilms, the extracellular polysaccharide content of 269BolA + ∆Yeaj (bolA overexpression and yeaJ deletion) was reduced by 89.91 % compared with ...