2017年8月17日 · G9a, a H3K9 methyltransferase, shows elevated expression in many types of human cancers, particularly breast cancer. However, the tumorigenic mechanism of G9a is still far from clear. Here we...

We demonstrated that G9a interacts with YY1 and is recruited to the JAK2 promoter along with corepressors, including histone deacetylase, that induced H3K9-me2. Repression of JAK2 transcription by G9a decreased H3Y41 phosphorylation and promoted inhibition of the recently identified JAK2-H3Y41P-HP1α pathway-mediated leukemogenesis.

2017年11月22日 · 首先作者分析了g9a和heph结合序列，在该序列中发现了yy1的两个结合位点。 YY1是一种转录因子，在不同的条件下可能激活也可以抑制蛋白表达。 作者发现敲低YY1可上调HEPH表达，同时敲低YY1及G9a可协同上调HEPH（图a）。

机制研究结果揭示，G9a与转录因子YY1和组蛋白去乙酰化酶HDAC1形成转录抑制复合物共同作用于在细胞铁稳态维持中起重要作用的亚铁氧化酶（hephaestin， HEPH）的启动子区，抑制该蛋白表达，进而使细胞内亚铁离子含量上调，促进乳腺癌生长。

We demonstrated that G9a interacts with YY1 and is recruited to the JAK2 promoter along with corepressors, including histone deacetylase, that induced H3K9-me2. Repression of JAK2 transcription by G9a decreased H3Y41 phosphorylation and promoted inhibition of the recently identified JAK2-H3Y41P-HP1α pathway-mediated leukemogenesis.

2017年8月17日 · G9a, a H3K9 methyltransferase, shows elevated expression in many types of human cancers, particularly breast cancer. However, the tumorigenic mechanism of G9a is still far from clear. Here we report that G9a exerts its oncogenic function in breast cancer by repressing hephaestin and destruction cellular iron homeostasis.

2015年4月20日 · Here, we provide evidence that UHRF1 is transcriptionally downregulated by H3K9 HMTase G9a. We found that increased expression of G9a along with transcription factor YY1 specifically represses UHRF1 transcription during TPA-mediated leukemia cell differentiation.

该研究工作揭示了HEPH表达受G9a调控的详细机制，为G9a在乳腺癌发展中的作用提供了新的解释，也为该类抑制剂的可能临床应用提供了有力支持。

2015年4月4日 · We found that increased expression of G9a along with transcription factor YY1 specifically represses UHRF1 transcription during TPA-mediated leukemia cell differentiation. Using ChIP analysis, we found that UHRF1 was among the transcriptionally silenced genes during leukemia cell differentiation.

2017年8月23日 · 该研究工作揭示了HEPH表达受G9a调控的详细机制，为G9a在乳腺癌发展中的作用提供了新的解释，也为该类抑制剂的可能临床应用提供了有力支持。