These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad …

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair …

ZFN dimers induce targeted DNA double-strand breaks (DSBs) that stimulate DNA damage response pathways. The binding specificity of the designed zinc-finger domain directs the ZFN …

A ZFN is an artificial endonuclease that consists of a designed zinc finger protein (ZFP) fused to the cleavage domain of the Fok I restriction enzyme. A ZFN may be redesigned to cleave new...

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to …

Zinc finger nucleases (ZFN) are one of the popular gene editing methods, consisting of a chimeric programmable nuclease containing a DNA-binding zinc finger domain and a nonspecific FOKl …

2025年2月12日 · At the molecular level, ZFNs are chimeric proteins composed of two functional domains: a DNA-binding domain and a nuclease domain. The DNA-binding domain is derived …

2019年3月8日 · These new architectures feature the functional attachment of the FokI cleavage domain to the amino terminus of one or both zinc-finger proteins (ZFPs) in the ZFN dimer, as …

2025年2月11日 · Explore the intricate role of ZFNs in genome editing, focusing on their unique protein architecture and precise DNA interaction. Zinc finger nucleases (ZFNs) have …

2005年9月23日 · Zinc finger nuclease (ZFN)-mediated gene targeting is rapidly becoming a powerful tool for “gene editing” and “directed mutagenesis” of plant and mammalian genomes …