Zip Nucleic Acids (ZNA®) are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA® are oligonucleotides conjugated to cationic units. ZNA® show increased affinity for their targets by reducing the electrostatic repulsion …

Despite sharing similar properties to LNA and MGB in terms of higher target affinity (increased Tm), ZNA® has unique features, such as higher Tm for short probes, which enhances discrimination (SNP genotyping, miRNA detection) and can be used to reduce the cycling time or improve the accuracy of quantification of low abundant transcripts, as ...

2019年9月13日 · To our best knowledge, we introduce a new generation nucleic acid “ZNA” probe based biosensor for the first time in this present work. It was developed for the detection of single nucleotide mutation related to FV Leiden in combination with …

ZNA®s enable specific and sensitive reactions when used as primers for PCR and Reverse Transcription (Figure 3-5). Moreover, ZNA® probes provide broad flexibility in assay design and represent an effective alternative to Minor Groove Binder (MGB)- and Locked Nucleic Acid (LNA)-containing oligonucleotides (3; 4).

2020年10月6日 · Objective: To investigate the performance of ZNA(ZIP Nucleic Acid) probes and its application in the quantitative detection of Chlamydia trachomatis (CT)nucleic acid. Methods: Use CT positive plasmids to compare the PCR amplification curves of …

Objective:To investigate the performance of ZNA(ZIP Nucleic Acid) probes and its application in the quantitative detection of Chlamydia trachomatis (CT)nucleic acid. Methods:Use CT positive plasmids to compare the PCR amplification curves of …

ZNA 結購是在DNA序列外側加上帶正電荷的精胺基團(spermine unit，也稱為Z unit)，正電荷結構幫助減少ZNA與互補序列間的負電排斥力，嵌住互補序列並形成狀似拉鍊結構，如下方作用機制圖示，因此被命名為Zip Nucleic Acid。

ZNA® (Figure 1) stands for Zip Nucleic Acid, which consist in oligonucleotides conjugated with cationic spermine units. This asset decreases the electrostatic repulsion between single strand nucleic acids (i.e. between primer/probe and target DNA during annealing).

摘要 目的:探讨ZNA (ZIP Nucleic Acid)探针的PCR性能及其在沙眼衣原体(CT)核酸定量检测中的研究。方法:使用CT阳性质粒,比较偶联不同ZIP数量的ZNA探针的PCR扩增曲线差异;比较ZNA探针与29me...

2019年9月13日 · In the present study, an impedimetric biosensing platform based on zip nucleic acids (ZNA) was developed for the sensitive detection of Factor V Leiden (FV Leiden) mutation.