Zip Nucleic Acids (ZNA®) are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA® are oligonucleotides conjugated to cationic units. ZNA® show increased affinity for their targets by reducing the electrostatic repulsion …

Despite sharing similar properties to LNA and MGB in terms of higher target affinity (increased Tm), ZNA® has unique features, such as higher Tm for short probes, which enhances discrimination (SNP genotyping, miRNA detection) and can be used to reduce the cycling time or improve the accuracy of quantification of low abundant transcripts, as ...

The possibility of modulating the global charge of the ZNA® oligonucleotide by the number of cationic spermine moieties attached to the nucleic acid oligomer is key to easily predict the melting temperature of ZNA®-DNA/ZNA®-RNA hybrids. Tm increases linearly with the length of the oligocation (Figure 2; 1).

Zip Nucleic Acid (ZNA) 為一種新型核苷酸修飾物。 ZNA 結購是在DNA序列外側加上帶正電荷的精胺基團 (spermine unit，也稱為Z unit)，正電荷結構幫助減少ZNA與互補序列間的負電排斥力，嵌住互補序列並形成狀似拉鍊結構，如下方作用機制圖示，因此被命名為Zip Nucleic Acid。

The Tm of ZNAs® increases linearly with the length of the oligocation. Therefore, it is possible to easily predict the melting temperature (Tm) of ZNA®-DNA or ZNA®-RNA hybrids. Figure 1. Structure of one single 5'-ZNA (R = sequence). metabion offers …

The approximate Tm value of ZNA oligonucleotide can be calculated using the formula “Tm (ZNA™) = Tm (DNA) + 36z/ (N-3.2)“, where z and N represent the number of cationic units and the number of nucleotides, respectively.

ZNA® are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA are oligonucleotides conjugated to cationic units. Their global charge can be modulated by the number of cationic spermine moieties grafted on each oligonucleotide.

ZNA® are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA are oligonucleotides conjugated to cationic units. Their global charge can be modulated by the number of cationic spermine moieties grafted on each oligonucleotide.

2015年9月23日 · Sigma-Aldrich Signs License Agreement With Polyplus-Transfection® to Offer Novel ZNA (TM) Oligonucleotides. Commercialization of ZNA™ technology provides for increased affinity for nucleic acids

In the current study, we used Zip Nucleic Acids (ZNA) as probes to increase its stability and melting temperature. Our aim was to genotype the -265 T/C changes of Apolipoprotein A-2 gene. We set up the real-time PCR reaction with ZNA probes, and by repeating the reactions, we confirmed the reliability of this new approach.