2023年8月3日 · ZsGreen荧光蛋白是1999年发表的一种碱性（本构荧光）绿色荧光蛋白，来源于Zoanthus sp.。 ZsGreen荧光蛋白激发波长(EX)是496nm，发射波（EM）是506nm。 ZsGreen荧光蛋白是用蓝光激发，发出绿色荧光。

ZsGreen is a basic (constitutively fluorescent) green fluorescent protein published in 1999, derived from Zoanthus sp..

Zoanthus green fluorescent protein, also known as zFP506. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. * Blocked by Dam methylation. Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present. * Blocked by Dam methylation.

Ai6 mice express robust ZsGreen1 fluorescence following Cre-mediated recombination.

2024年8月26日 · ZsGreen 来源于造礁珊瑚，是四聚体，和GFP相比，序列差异大(EGFP大小720bp， ZsGreen大小696bp)，ZsGreen 具有更强更稳定的荧光，但不适合和目的基因融合。

ZsGreen1 is the brightest commercially available green fluorescent protein—up to 4X brighter than EGFP—and is ideally suited for whole-cell labelling, promoter-reporter studies, or as a transfection control. Cat.

ZsGreen 属于RCFP荧光蛋白家族，荧光信噪比最高。 使用pET28构建载体pET28ZsGreen；以 pT7 载体构建pT7-ZsGreen 最终表达载体。 使用 Gateway 克隆方法将目的蛋白人雌二醇受体 DNA 结合区域（ERDBD）、R183-H267 和人野生型 EphB2 蛋白克隆到最终表达载体 RCFP 序列上 …

After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility. BtgZI is typically used at 60°C, but is 75% active at 37°C. BsrGI is typically used at 37°C, but is even …

ZsGreen1 is a basic (constitutively fluorescent) green fluorescent protein, derived from Zoanthus sp..

2022年8月13日 · ZsGreen荧光蛋白是用蓝光激发，发出绿色荧光。 ZsGreen1且在505纳米处具有发射峰的珊瑚礁珊瑚蛋白已被引入，以替代增强型绿色荧光蛋白。 当在哺乳动物细胞中表达时，ZsGreen1相对于 EGFP 明亮，但在产生融合突变体方面的作用有限，并且与其他珊瑚礁蛋白类 …