In this study, a workflow was developed for the consistency monitoring and biochemical marker identification of the commercial-scale CHO cell culture process through 1 H NMR assisted with multivariate data analysis (MVDA).

2021年12月16日 · As seen in the 1 H NMR spectrum of methyl acetate (Fig. 6.6a), the x-axis units of NMR spectrum are in ppm (not in Hz as we would expect for frequency), and the two signals stand at different position along the x-axis. Let’s explain how that works and what information can be obtained.

2023年4月27日 · In this study, a workflow was developed for the consistency monitoring and biochemical marker identification of the commercial-scale CHO cell culture process through 1 H NMR assisted with multivariate data analysis (MVDA).

The structure and function of the chemicals contributing to the three main peaks seen with 1 H NMR spectroscopy, N-acetyl-L-aspartate (NAA), creatine/phosphocreatine (Cr), and choline-containing compounds (Cho) is reviewed and the changes seen with these compounds in various disease states are briefly outlined. NAA is present within neurons ...

2018年1月15日 · The current NMR study gives experimental results for 1 H and 13 C nuclear magnetic shielding of 13 C double enriched acetaldehyde for the isolated molecule: σ 0 (CH 3) = 28.863 ppm, σ 0 (CHO) = 21.072 ppm, σ 0 (CH 3) = 156.75 ppm and σ 0 (CHO) = −6.71 ppm.

核磁共振氢谱 (也称 氢谱, 或者 1H 谱) 是一种将 分子 中 氢-1 的 核磁共振 效应体现于 核磁共振波谱法 中的应用。 可用来确定分子结构。 [1] 当样品中含有氢，特别是同位素氢-1的时候，核磁共振氢谱可被用来确定分子的结构。 氢-1原子也被称之为氕。 简单的氢谱来自于含有样本的溶液。 为了避免溶剂中的质子的干扰，制备样本时通常使用 氘代溶剂 （氘= 2 H， 通常用D表示），例如： 氘代水 D 2 O， 氘代丙酮 (CD 3) 2 CO， 氘代甲醇 CD 3 OD， 氘代二甲亚砜 (CD 3) 2 SO和 …

This study demonstrates the potential of employing 1 H NMR-based metabolomics technology in bioprocess research. It provides valuable guidance for feed medium development, feeding strategy design, bioprocess parameter adjustments, and ultimately the enhancement of cell proliferation and mAb yield.

The structure and function of the chemicals contributing to the three main peaks seen with 1H NMR spectroscopy, N-acetyl-L-aspartate (NAA), creatine/phosphocreatine (Cr), and choline-containing compounds (Cho) is reviewed and the changes seen with these compounds in various disease states are briefl …

2022年12月6日 · In this study, a workflow was developed for the consistency monitoring and biochemical marker identification of the commercial-scale CHO cell culture process through H NMR assisted with...

为了鉴定化合物(cho)结构,应用核磁共振一维谱(h-nmr)和c-nmr确定了化合物中基团类型,并用二维相关谱(h-hnmr和h-cnmr)中的偶合关系,找到了各原子间的连接信息,对dept谱中c谱线进行归属,用化学位移和偶合常数的方法确定了分子中不饱和双键质子的顺反构型,最后确定了 ...