In gene therapy, minimization and removal of double-stranded RNA (dsRNA) is important for mRNA in vitro transcription (IVT) processes. Anti-dsRNA monoclonal antibody J2 is a useful tool in dsRNA detection. J2 antibody also can be used to detect dsRNA intermediates of Hepatitis C virus, Dengue virus, rhinovirus and Chikungunya virus.

Double-stranded RNA (dsRNA) is a viral product that induces innate immunity, leading to the production of interferon (IFN) alpha and beta, which can lead to the activation of hundreds of IFN-stimulated genes that confer resistance to viruses. dsRNA of more than 30-bp length are reported to be is a key activator of the innate immune response against viral …

Antibody J2 (1:250 dilution, 4 microgram/ml) reveals the colocalisation of dsRNA (labelled in green) and Hepatitis C virus core protein (labelled in red), indicating the location of putative virus assembly sites (arrows).

J2抗体是一种抗dsRNA抗体，可特异性识别并结合dsRNA，根据特定信号鉴别样品中是否存在dsRNA（定性检测），如图2C所示。 同时制备合适的标准对照品，以定量分析待测样品的dsRNA残留，提高检测的灵敏度（图2E）。

2022年10月31日 · 检测具有高度特异性：在存在 1.000-10.000 倍过量其他核酸的情况下，可以检测核酸提取物中的dsRNA。该测定基于双抗夹心ELISA原理，使用 J2 (IgG2a) 小鼠单克隆抗体作为捕获抗体，单克隆抗体 K2 (IgM) 用作检测抗体。 双链RNA (dsRNA)抗体包括J2、K1、K2和J5

dsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The J2 clone shows a higher affinity to natural dsRNA than to other dsRNA antigens (6).

Double-stranded RNA (dsRNA) ELISA Kit (J2 based) is a Sandwich ELISA assay with two kinds of the monoclonal antibodies (J2) to sensitively and selectively detect dsRNA structure (larger than 30-40 bp) in mRNA molecules, independent of their nucleotide composition and sequence.

SCICONS Anti-double-stranded RNA Monoclonal (J2), Catalog # 10010200-200UG. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC), ELISA (ELISA) and Affinity Purification (AP) applications. This antibody reacts with Chemical samples. Supplied as 200 µg purified antibody.

Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy.

Recombinant monoclonal antibody to dsRNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma J2. Immunogen: The original J2 antibody was raised by immunising mice with L-dsRNA. Specificity: This …