Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3.

pGEX-4T-1载体价格800元，pGEX4T1载体质粒图谱(Vector map),pGEX 4T 1载体列(Sequence)和多克隆位点见下文，载体抗性为氨苄，质粒大小为4969 bp，测序引物为pGEX5'和pGEX3'，主要用于大肠杆菌蛋白表达.

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thrombin or factor Xa protease sites to cleave protein from fusion. pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs. Hosts: E.coli. Related vectors: pGEX-2T. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.) Catalog Number 27458001 …

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pGEX-4T-1载体是一种常用的原核表达载体，具有 Amp 抗性，质粒大小为 4.9kb 。 该载体多克隆位点区 域含有多个常用的内切酶位点序列，便于不同基因的克隆；且全序列中含有 tac 启动子、 GST 标签序列，

Plasmid pGEX-4T1 SHP2 WT from Dr. Ben Neel's lab contains the insert SHP2 and is published in Mol Cell Biol 2000 Jan;20 (1):299-311. This plasmid is available through Addgene.

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site. pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries. A tac promoter for chemically inducible, high-level expression of GST-tagged recombinant Proteins.

2023年12月22日 · 产品名称： pgex-4t-1 别称pgex4t1；pgex4t-1. 特征特性： 宿主: 大肠杆菌,片段类型: orf,片段物种: 空载体,原核抗性: amp,启动子: tac,复制子: pbr322,拷贝数:低,感受态: dh5a,温度:37度,正向引物:pgex-5':gggctggcaagccacgtttggtg,反向引物:pgex-3':ccgggagctgcatgtgtcagagg,诱导方式: iptg. 主要 ...

Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-4T-1 using any one, or combination of unique restriction sites and transform into a host of choice such as E. coli BL21 (27154201).