pGEX-6P-1载体价格800元，pGEX6P1载体质粒图谱(Vector map),pGEX 6P 1载体列(Sequence)和多克隆位点见下文，载体抗性为氨苄，质粒大小为4984 bp，测序引物为pGEX5'和pGEX3'，主要用于大肠杆菌蛋白表达.

Bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P-3.

pGEX系列载体，该系列载体利用 tac启动子 ，在tac启动子和多克隆位点之间插入了两个有利于纯化的编码序列，其一是 谷胱甘肽巯基转移酶 基因，其二是编码 凝血蛋白酶 或者 Factor Xa因子 （简称Xa因子）切割位点的序列。这样，外源基因表达时会表达出编码三个 ...

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The pGEX-6P-1 is a bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P-3.

PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product. Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites.

Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-6P-1 using any one, or combination of unique restriction sites and transform into a host of choice such as E. coli BL21 (27154201).

pGEX-1_lambda_T. pGEX-4T-1. pGEX-5X-1. pGEX-6P-1. pGEX-2T. pGEX-4T-2. pGEX-5X-2. pGEX-6P-2. pGEX-2TK. pGEX-4T-3. pGEX-5X-3. pGEX-6P-3. pGEX-3X. Plasmid Sets. Basic Cloning Vectors. I.M.A.G.E. Consortium Plasmids. pET & Duet Vectors (Novagen) TA and GC Cloning Vectors. Coronavirus Resources. Insect Cell Vectors.

2024年3月27日 · pGEX-4T-1载体是一种常用的原核表达载体，具有Amp抗性，质粒大小为4.9kb。 该载体多克隆位点区域含有多个常用的内切酶位点序列，便于不同基因的克隆；且全序列中含有tac启动子、GST标签序列，GST标签位于融合基因的N端，是一种高效的蛋白表达载体。

Plasmid pGEX-GP-2-ATRX ADD from Dr. Albert Jeltsch's lab contains the insert ATRX ADD and is published in Genome Res. 2014 Nov;24 (11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9. This plasmid is available through Addgene.