The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C …

包括p65的磷酸化调节和p65的乙酰化调节、p53对转录的竞争性调节，gsk3β对转录的间接调节；nf-κb转录调节中的常见的激酶：pka（蛋白激酶a），msk1和msk2（分裂素和压力激活激酶 ）。

In resting cells the PKA catalytic subunit (PKA-C) is bound in an inactive state to cytosolic IκBα: p65 complexes. Following IKK complex activation and the degradation of IκBα, active PKA-C is liberated and phosphorylates p65 at S276 in a cAMP-independent process [55,56].

1998年4月1日 · Phosphorylation by PKA both weakens the interaction between the N- and C-terminal regions of p65 and creates an additional site for interaction with CBP/p300. Therefore, PKA regulates the transcriptional activity of NF-κB by modulating its interaction with CBP/p300.

2021年1月1日 · Furthermore, PKA can phosphorylate the NF-κB subunit p65, a transcription factor that is essential for T-cell activation, independently of cAMP. This phosphorylation of p65 drastically enhances NF-κB-dependent transcription and …

1997年5月2日 · Dephosphorylation of nuclear extracts from induced cells reduces the DNA-binding activity of NF-κB in vitro. In addition, PKA can phosphorylate the recombinant p65–p65 homodimer and p65–p50 heterodimer, leading to an enhancement of their DNA-binding activity.

Phosphorylation by PKA both weakens the interaction between the N- and C-terminal regions of p65 and creates an additional site for interaction with CBP/p300. Therefore, PKA regulates the transcriptional activity of NF-κB by modulating its interaction with CBP/p300.

2025年2月16日 · In MH-S cells, luciferase analysis indicated that PDE4B acts as a positive regulator of p65 in inflammation. PKA inhibitor (H-89) increased pP65 and PDE4B expression, while PKA activator (6-BZ-cAMP) showed the opposite effect in macrophages.

In this report, we demonstrate that PKA activation in Schwann cells leads to phosphorylation of p65 at serine 276, and this increases its transcriptional activity. This phosphorylation of NF-κB was observed in both myelinating cocultures and developing sciatic nerves.

2009年10月1日 · Here, we investigated the prevalence and function of p65-Ser276 phosphorylation by protein kinase A (PKA) in the malignant phenotype and gene transactivation, and studied p65-Ser276 as a potential target for therapy.