Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends …

pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. [1] The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") …

Plasmid pUC19 from Dr. Joachim Messing's lab is published in Gene. 1983 Dec;26 (1):101-6. This plasmid is available through Addgene.

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 …

2023年8月3日 · It is a commonly used cloning vector in the bacteria E. coli. pUC19 is 2686 bp in length. The molecular weight of the pUC19 vector is 1.75×10 6 Da. It is a small plasmid with a …

Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in …

pUC19 is a small, high-copy number E. coli plasmid cloning vector containing portions of pBR322 and M13mp19 (1). It con-tains the pMB1 origin of replication from pBR322, but it lacks the rop …

pUC19 DNA: Location of Sites PmlI, PpuMI, PshAI, PsiI, PspOMI, PspXI, RsrII, SacII, SanDI(x), SexAI, SfiI, SgrAI, SnaBI, SpeI, SrfI(x), StuI, StyI, SwaI, TliI ...

2024年10月26日 · The pUC19 cloning vector is a widely used tool in molecular biology, valued for its efficiency and versatility in genetic engineering. Its compact design allows researchers to …

2024年6月6日 · The pUC19 plasmid, developed by Joachim Messing and colleagues, is a widely utilized cloning vector in E. coli bacteria. With a length of 2686 base pairs and a molecular …

Linearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit.

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. …

After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility. For full activity, add fresh S-adenosylmethionine (SAM). Efficient cleavage requires at least two copies …

pBR322 and pUC19 are plasmids that are used as cloning vectors. The key difference is their molecular size and base pair length. Let’s look at the other differences between pBR322 and …

The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector).

2009年5月29日 · We report here the identification of a putative gene cluster involved in the biosynthesis, regulation and resistance of A-500359s. Using reverse transcriptase PCR (RT …

pUC19 is a commonly used E. coli cloning vector. It is a small double-stranded DNA circle, 2686 base pairs in length, and has high copy number. pUC19 has a 54 base pair polylinker that …

We have cloned a protective M-like protein gene (SzPW60) of an equine strain of S. equi subsp. zooepidemicus W60 and determined its sequence.

To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III translocon to microinject several anti-host Yop effectors into the cytosol of target eukaryotic …

Flotillins are lipid raft-associated proteins, which have been implicated in neuronal regeneration and in-sulin signaling. We now show that newly synthesized flotillin-1 reaches the plasma …