Biomark™ HD is an automated, high-performance PCR system that uses microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, sample identification, copy number variation analysis and digital PCR. Ideal for labs performing PCR on hundreds to thousands of samples, the Biomark HD provides significant

Quantitative PCR (qPCR) is a technique commonly employed in laboratories and core facilities. In our previous study, we had shown the possibility to automate steps in a panel-specific gene expression workflow by pairing Mosquito HV with BioMark HD. Here we aimed to automate the full workflow and explore miniaturization capabilities.

2019年4月24日 · We aimed to develop a novel nanofluidic real-time PCR (Fluidigm) assay that simultaneously detected and quantified 11 bacterial pathogens in 96 different samples (92 samples and 4 controls) in a...

qPCR，全称Quantitative Polymerase Chain Reaction，即定量聚合酶链式反应，是一种常见且较为重要的实验室技术，可以对目标DNA或RNA进行定性以及定量分析。 与传统的PCR不同，qPCR不仅能扩增DNA，还能实时监测扩增过程中的荧光信号变化，进而得出目标序列在样本中的数量。 qPCR的基本原理是在PCR反应中，通过添加 荧光染料 或 荧光探针，当DNA模板被扩增时，荧光信号会随之增加。 实时监测荧光信号的变化可以计算出扩增曲线，从而精确地得出 …

We aimed to develop a novel nanofluidic real-time PCR (Fluidigm) assay that simultaneously detected and quantified 11 bacterial pathogens in 96 diferent samples (92 samples and 4 controls) in a...

2023年3月30日 · Real Time PCR，即实时荧光定量PCR，也被称为定量PCR或qPCR，是实时监测PCR扩增产物并进行解析的方法。 由于定量PCR具有操作简单、快速方便、灵敏度高、重复性好、污染率低等优点，被广泛应用于医学检测、药物疗效考核、基因表达研究、转基因研究、基因 …

pcr、qpcr、rt-pcr和rt-qpcr这四种技术的主要区别在于它们的应用领域和检测目标不同。 PCR主要用于DNA序列的扩增和检测；qPCR可以实时定量检测DNA或cDNA的含量；RT-PCR主要用于RNA分子的扩增和检测；而RT-qPCR则结合了RT-PCR和qPCR的技术，可以对RNA分子进行定量 …

The BioMark HD is an automated, high-performance PCR/qPCR system that uses microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, sample identification, copy number variation analysis and digital PCR.

The high-throughput quantitative PCR platform is equipped with Fluidigm’s Biomark-HD™, a system that allows the simultaneous quantification of the expression of tens of genes in a large number of samples in microfluidic chips. Each chip consists of small volume reaction chambers (0.85nL, 6nL or 9nL) and an integrated fluidic circuit (IFC).

2016年6月2日 · High throughput qPCR platform raises several questions in terms of sensitivity (minimal input), absolute quantification and data processing. To address these questions, we designed experiments...