Our real-time PCR kits help you overcome these challenges and demonstrate high specificity thanks to a novel hot-start mechanism, allowing you to detect target sequences down to one copy – plus, HRM solutions are available.

Real-time PCR or quantitative PCR (qPCR) enables sensitive, specific detection and quantification of nucleic acid targets. A powerful and versatile tool, qPCR supports an array of applications from gene expression to pathogen detection to genotyping.

QuantiTect PCR Kits (qPCR kits) enable sensitive quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using sequence-specific probes or SYBR Green I detection. The real-time PCR kits also allow reliable quantification of up to 5 gDNA or cDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR.

iTaq Universal One-Step RT-qPCR Kits provide high PCR efficiency in sensitive SYBR ® Green or probe assays, even with difficult targets and challenging samples. Ideal for low-to-mid-throughput RT-qPCR screening. Earn a free one-year service contract on your CFX System with purchase of Reagents.

Explore an array of reagents for dye- and probe-based qPCR. Multiplexing capability. Supermixes for reliable genomic DNA, cDNA, and plasmid DNA amplification.

Reverse Transcription and qPCR in One Reaction. The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well.

qPCRBIO SyGreen® 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube. Ready-to-use real time master mixes for all dye-based and probe-based qPCR and RT-qPCR applications.

These kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g., TaqMan ®), FRET probes, and displacement probes (e.g., molecular beacons).

Optimized real-time PCR (qPCR) analysis enables sensitive and specific quantification of nucleic acids. Reagent selection plays a critical role in any qPCR or RT-qPCR protocol to help ensure optimal performance and reliable results.

We offer one-step RT-qPCR kits supporting both TB Green- (our proprietary green intercalating dye) and probe-based chemistries to meet your experimental needs and give you the flexibility to run a wide variety of applications.

QuantiNova Multiplex PCR Kits (multiplex qPCR kits) enable fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote ultrafast multiplex real-time PCR within 1 …

Our PCR kits with unique QIAGEN PCR Buffer and highly stringent PCR enzymes eliminate the need for PCR optimization

Discover QuantiNova Kits – Today’s most advanced portfolio of real-time PCR and RT-PCR kits for gene expression analysis, gene quantitation and pathogen detection. Experience the advantages of our unique, integrated controls and next-generation procedural improvements that provide increased precision, and reproducibility and efficiency ...

Kits for RT-qPCR, or quantitative reverse transcriptase PCR or real-time RT-PCR, enable quantitative PCR directly from RNA samples. These ready-to-use kits include the necessary components for carrying out both cDNA synthesis and qPCR.

NovaQUANT™ quantitative real-time PCR (qPCR) assays are user-friendly kits to determine ratios of mitochondrial to nuclear DNA or mRNA levels of key genes associated with mitochondrial biogenesis and oxidative stress.

Discover Agilent’s range of qPCR specialty kits and reagents, like the MycoSensor qPCR assay kits, the miRNA qRT-PCR detection reagents, the porcine detection kit, and the qPCR NGS library quantification kit.

Our real-time PCR master mixes provide exceptional value by combining high-performance ingredients with user-friendly features. They are formulated to deliver superior sensitivity, specificity, and reproducibility, making them suitable for a wide range of applications from basic research to complex assays.

Microbial DNA qPCR Assay Kits contain a Microbial DNA qPCR Assay, which is a mix of two PCR primers (10 µM each) and one 5′-hydrolysis probe (5 µM) that enables quantitative real-time PCR for 20 reactions.

We have specialized kits for a variety of PCR, qPCR, and RT-PCR applications throughout your PCR workflow. Additionally, explore our comprehensive offering of PCR-related resources, including the Standard PCR Protocol and PCR Master Mix Calculator tool. Explore our robust offering of PCR reagents and kits.

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment. Legal Information.

Our qPCR kits for use with qPCR probes have a unique combination of in-process controls – including a built-in visual indicator – to remove variables and ensure precise reaction setup. Thanks to a novel hot-start mechanism, our kits demonstrate high specificity and sensitivity, allowing you to detect target sequences down to one copy.

2025年3月13日 · Use high-quality RNA/DNA extraction kits designed to minimize inhibitors. Perform additional purification steps (e.g., ethanol precipitation or column-based clean-up) for complex samples. Dilute the template to reduce inhibitor concentration, ensuring that the target remains detectable. 2. Optimize qPCR Reaction Conditions