2020年7月15日 · We reveal a nucleolar Pol-II-dependent mechanism that drives ribosome biogenesis, identify disease-associated disruption of nucleoli by noncoding RNAs, and establish locus-targeted R-loop...

2024年11月6日 · Ribosomal DNA (rDNA) repeats harbor ribosomal RNA (rRNA) genes and intergenic spacers (IGS). RNA polymerase (Pol) I transcribes rRNA genes yielding rRNA components of ribosomes. IGS-associated...

2006年5月5日 · Transcripts originating from the intergenic spacer (IGS) that separates rRNA genes (rDNA) have been known for two decades; their biological role, however, is largely unknown. Here we show that IGS transcripts are required for establishing and maintaining a specific heterochromatic configuration at the promoter of a subset of rDNA arrays.

2013年10月31日 · The large intergenic spacer (IGS) region of rDNA is a valuable DNA sequence because it contains the signals for transcription and processing of rRNAs. In this study, we sequenced and analyzed the complete nucleotide of rDNA IGS …

2021年1月1日 · The sequence of the rRNA genes is highly conserved among eukaryotes. However, the intergenic spacer regions (IGS) is quite divergent in length and sequence but carry three unique regulatory regions including an origin of replication, a replication fork barrier site and a promoter that drives the expression of noncoding RNAs (Fig. 3.2 B and C

2017年8月17日 · In most plants, due to the differences in the SR sequences (including copy number, sequence, and length) of the IGS region among different species, the IGS region may provide a more suitable marker for reconstructing phylogenetic relationships within both inter- and intra-species than the 45 rDNA ITS and 5S rDNA NTS.

2023年7月4日 · In eukaryotes, rDNAs form a multi-copy family of sequences organized in tandem repeats across one or several loci. The individual genes are separated by internal transcribed (ITS1 and ITS2) and...

2003年12月1日 · Here we report the sequencing of the mouse IGS from a single bacterial artificial chromosome (BAC) clone. The sequencing of the IGS allowed us to assemble one complete rDNA unit. We also consider the role of various IGS elements in transcription regulation, replication, and scaffold binding.

2018年12月5日 · These new sequences were used to determine the conservation profile of the human rDNA, revealing 49 conserved regions in the rDNA intergenic spacer (IGS). To provide insights into the potential roles of these conserved regions, the conservation profile was integrated with functional genomics datasets.

2019年4月30日 · The 35S ribosomal DNA (rDNA) units that occur in tandem repeat are separated by an intergenic spacer (IGS) that plays an important role in rRNA transcription. Moreover, IGS is an important molecular marker for evolutionary research in plants.