2023年4月19日 · We designed a novel strategy, referred to as stapling, that introduces two disulfide bonds between the scFv linker and the two variable domains to minimize scFv breathing. We named the resulting molecules stapled scFv (spFv). Stapling increased thermal stability (Tm) by an average of 10°C.

Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells. However, scFv domains exhibit lower …

We designed a novel strategy, referred to as stapling, that introduces two disulfide bonds between the scFv linker and the two variable domains to minimize scFv breathing. We named the resulting molecules stapled scFv (spFv). Stapling increased thermal stability (Tm) by an average of 10°C.

ScFv，全称为“Single-Chain Fragment Variable”，中文翻译为“单链可变区片段”，是一种基因工程融合蛋白 [1]，是由抗体重链的可变区与轻链的可变区在一段肽链（linker，后文有详细介绍）的连接下构成的小分子（图1） [2]。 图1：抗体模型：亚基的组成以及沿多肽链的domain分布，ScFv抗体由抗体重组技术得到。 Fv片段是具有抗原结合活性的最小的免疫球蛋白分子单位。 抗体片段（antibody fragment）也可与一系列小分子相融合（fuse） [3]，包括用于癌症成像的放射性同 …

Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen...

Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen...

scFV：为了使Fv更加稳定，用一段含4～25个氨基酸的linker将VL和VH连接起来，形成单链抗体（scFv）。 单区（域）抗体 是指具有抗原结合活性的抗体亚单位，一般是指单独的重链可变区，但通常亲和力低，非特异性结合明显增加。

scFv具有分子质量小、穿透力将强、特异性较高等优点，在靶向治疗、影像诊断、细胞内免疫、生物检测等方面有 着重要的应用。 在靶向治疗方面，可以将药物和毒素与scFv链接，形成免疫药物或者免疫毒素等，利用抗原与抗

随着基因工程抗体的研发，scFv（single-chain fragment variable）在理论和实际应用方面的研究引起了越来越多的关注。 与经典抗体分子相比，scFv具有分子量小（约占完整抗体分子的1/6）的特征，最早是在酵母和植物…

2023年4月23日 · The “stapled” scFv molecules (spFv) generally have an increase of about 10 ºC in Tm for scFv molecules with both kappa and lambda light chains. In a set of anti-CD3 scFv/spFv and anti-BCMA Fab bispecific molecules, the results show that stapling the anti-CD3 scFv significantly improved the yields and quality of the bispecific monomer ...