SOLVED: You are studying the expression of your favorite gene, YFG1. You have cloned the entire gene, including its promoter, into a plasmid. In addition to the wild type, you have also made mutations in two different parts of the gene that you suspect are in the promoter. Let's refer to these mutants as Mut1 and Mut2. After transforming these three plasmids into separate cultures of cells, describe an assay you could use to measure the amount of RNA produced and how you would determine if Mut1

Improving protein expression in E. coli B-strains using rapid genome engineering