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Halo-Ligand Staining of Cells for Protein Condensate-Interaction Studies Using Single-Molecule Imaging

Halo-Ligand Staining of Cells for Protein Condensate-Interaction Studies Using Single-Molecule Imaging

已浏览 38 次6 个月之前

SOLVED: A quantitative study of the interaction of a protein with itsligand yielded the following results:Ligand concentration (nM)123456912v (moles of ligand bound per mole ofprotein)0.370.540.640.700.750.780.840.88Give the x- and y-axis you would useto plot the graph then determine the valueof KD, the dissociation constant for theinteraction between the protein and its ligand.(You will probably need to draw a graph to obtain a valuefor KD.)

SOLVED: A quantitative study of the interaction of a protein with itsligand yielded the following results:Ligand concentration (nM)123456912v (moles of ligand bound per mole ofprotein)0.370.540.640.700.750.780.840.88Give the x- and y-axis you would useto plot the graph then determine the valueof KD, the dissociation constant for theinteraction between the protein and its ligand.(You will probably need to draw a graph to obtain a valuefor KD.)

2022年12月22日

Verification of the Interaction Between Xanthatin and Keap1 Protein Using Western Blotting (Video) | JoVE

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