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PCR Primer Design for Cloning by Restriction Enzyme | Videos

PPT - PCR Primer Design PowerPoint Presentation, free download - ID:737169

DNA complementary to pepper mRNA was synthesized using oligo (dT) as a primer for the first synthesis. The second strand (synonymous with the mRNA) was then synthesized, and the population of double-stranded cDNAs were ligated into a plasmid vector using a procedure that leaves Pstl sites flanking the cDNA insert (i.e. the terminal Pstl sites for each clone are not part of the cDNA). This cDNA library was screened for clones made from the mRNA from the pepper. One clone was isolated, and subsequ

SOLVED: The original DNA sequencing procedure invented by Fred Sanger used a DNA template (the molecule whose sequence you wish to determine), a radioactively labeled oligonucleotide primer, DNA polymerase, deoxyribonucleotide triphosphates (dATP, dCTP, dGTP, and dTTP), and dideoxyribonucleotide triphosphates (ddATP, ddCTP, ddGTP, ddTTP). a) Explain why ddATP, ddCTP, ddGTP, and ddTTP were included in the sequencing procedure.b) What will happen if the researcher mistakenly adds ten times less di

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DNA Replication: Steps and Mechanism • Microbe Online

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