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Assertion (A): In Thalassemia an abnormal myoglobin chain is synthesized due to a gene defect. Reason (R): α-Thalassemia is controlled by genes HBA-1 and HBA-2 on chromosome 16. - Biology | Shaalaa.com
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SOLVED: There are two sequences listed above: One is the normal beta-globin allele (HbA) and one is the sickle cell beta-globin allele (HbS). To distinguish between the two sequences, we use the restriction enzyme DdeI. DdeI recognizes the following sequence: CINAGGANTK. Each nucleotide of the sequence could be any of the four DNA bases. In this case, "N" means that the middle sequences CTAAG, CTTAG, CTGAG, and CTCAG would all be cut by DdeI. The line indicates exactly where the cut happens in t
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