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SOLVED: Find kcat for a reaction in which Vmax is 4 × 10^-4 mol/min and the reaction mixture contains one microgram of enzyme (the molecular weight of the enzyme is 200,000 Da). Show your work/reasoning.
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SOLVED: Calculate the Km, Vmax, and Kcat for a molecular weight enzyme GOkDa from (final protein concentration in the following "data cuvette IOug/ml) with [S]; mM Vo points: Amol/min10 20 30Calculate Km and Vmax using both Michaelis-Menten data and Lineweaver-Burk equations from the following [S], mM Vo Mmol/min 0.1 0.34 0.2 0.53 0.4 0.74 0.8 0.91 1.6 1.04
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