Enzyme Activity: Biology Lab

Enzyme Activity Lab

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

SOLVED: How might you determine how much peroxidase is ACTUALLY in thevolume of extract used to create your linear curve? Thinkabout the experiments you might need to do. Refer to yourbiochemistry textbook if you need ideas.

Given below is a possible three-step mechanism describing the reaction of hydrogen peroxide with iodide ion in an acidic solution. Step 1: (slow) H2O2(aq) I- (aq) —> HOI (aq) Step 2: (very fast) OH- (aq) H (aq) —> H2O (liq) Step 3: (fast) HOI (aq) H (aq) I- (aq) —> I2(aq) H2O (liq) Assuming that the above reaction mechanism is correct, write the expected rate law expression. Is your experimental data consistent with the proposed mechanism? Explain your answer

Write a balanced half-reaction for the oxidation of liquid water (H2O) to aqueous hydrogen peroxide (H2O2) in acidic aqueous solution. Be sure to add physical state symbols where appropriate.

Alkene Epoxidation Reaction and Mechanism with mCPBA - MCAT and Organic Chemistry Study Guides, Videos, Cheat Sheets, tutoring and more

Redox Reaction | Overview, Application & Examples

A suggested mechanism for the decomposition of hydrogen peroxid... | Filo