DIGESTION

Process of Digestion: Digestion Process in Mouth, Stomach, Intestines

SOLVED: A DNA sequence 10 kb long contains one site at which it may be cut by the restriction enzyme PstI. The same 10 kb long sequence also contains one site that can be cut by the restriction enzyme BglII. Three separate fractions of this DNA were treated separately as follows (see figure below): digestion by PstI; digestion by BglII; and digestion by both enzymes. These three treated fractions were then subjected to gel electrophoresis. The following table shows the lengths of the restriction

SOLVED: You have a plasmid cloning vector that is 3000 bp in size. It has individual cloning/recognition sites for the enzymes EcoRI, BamHI, and PstI. Cloning a fragment of DNA into the BamHI site will disrupt the lacZ gene. This plasmid also has a gene conferring ampicillin resistance; this gene does not disrupt any of these restriction sites. You digest some of this plasmid vector with the enzyme BamHI. You also isolated some of the DNA from your pet bird, Bubba, using the cheek swab procedure

Gene Cloning using Plasmids: Molecular Cloning Intro - SciGine

DNA cloning protocol for gene therapy development