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SOLVED:The protein SmpB binds to a special species of tRNA. tmRNA, to eliminate the incomplete proteins made from truncated mRNAs in bacteria. If the binding of SmpB to tmRNA is plotted as fraction tmRNA bound versus SmpB concentration, one obtains a symmetrical S-shaped curve as shown in Figure Q 3-3. This curve is a visual display of a very useful relationship between Kd and concentration, which has broad applicability. The general expression for fraction of ligand bound is derived from the eq
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